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Common enzyme quantitative methods include enzyme
2021-05-31

Common enzyme quantitative methods include enzyme-linked immunosorbent assay [,] (ELISA), chemiluminescence (CLIA) [, , ] and immunochromatography []. The advantages of ELISA are cost reduction and automation, but non-specific reaction caused by HOOK effect, false positive caused by cross-contaminat
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br Materials methods br Results
2021-05-31

Materials & methods Results Discussion As shown by qPCR analysis, Bdnf1 expression was up-regulated by approximately fifty percent after injection of RG108. A possible explanation is that the overall methylation in promoter of exon I is decreased by the compound, therefore facilitating its
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Diacylglycerol kinase was one of the hits
2021-05-31

Diacylglycerol kinase was one of the hits identified from the RNAi screen. Diacylglycerol kinases are conserved across a Fluorescein TSA of species with more complex, multicellular organisms possessing several DGKs with differing protein domains, expression patterns and functions [61], [62], [63].
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wst-1 br Discussion Electroporation is a convenient and effi
2021-05-31

Discussion Electroporation is a convenient and efficient way to introduce foreign material into wst-1 [10], [16], [17], [18]. There are a lot of different protocols for transfection with electroporation. It was reported that there are several parameters that are relevant to successful electropor
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We have performed our MSP analyses as real time
2021-05-31

We have performed our MSP analyses as real-time PCR using SybrGreen (Fig. 2). The results of the beta-actin PCR vary in each sample group because the amount of DNA for bisulfite treatment was adjusted to a constant volume of serum (500μl) rather than to equal amounts of DNA. Because we expected lowe
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Cytochrome P CYP enzymes plays an essential role in pharmaco
2021-05-31

Cytochrome P450 (CYP-450) enzymes plays an essential role in pharmacology of drugs and toxicology of xenobiotics. Inhibition of these enzymes may lead to increase plasma levels of a concomitantly administered drug (Lee et al., 2013). It may prolong or reduce the pharmacological effects and increase
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Electroencephalographs at onset ranged from hypsarrhythmia t
2021-05-31

Electroencephalographs at onset ranged from hypsarrhythmia to mild abnormalities but more abnormalities in background rhythms and epileptiform activity develop over time.15, 17, 19, 48, 52, 54, 55 Early mild abnormalities that sometimes precede a diffuse encephalopathy included focal delta slowing i
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Similarly to the chemokine CCL the level of the chemokine
2021-05-31

Similarly to the chemokine CCL-5, the level of the chemokine SDF-1 in the groups of individuals with APD did not differ from its level in the control group. However, a slight increase in the level of this chemokine was recorded in the group of women and men with APD and PTSD as compared with the cor
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Different types of environmentally induced antinociception h
2021-05-31

Different types of environmentally induced antinociception have been reported in a wide range of species (e.g., Behbehani, 1995, Bolles and Fanselow, 1980, Harris, 1996, Millan, 2002, Rodgers, 1995). According to Bolles and Fanselow (1980), fear and pain are independent and competing motivational sy
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Hinokitiol has versatile abilities as anticancer antimicrobi
2021-05-28

Hinokitiol has versatile abilities as anticancer, antimicrobial and molecule transport agents and appears to have no developmental toxicity or carcinogenic effects (Imai et al., 2006b). For anticancer activities, it causes apoptosis and T7 High Yield Cy3 RNA arrest in many different types of cancer
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br Materials and methods br Results br Discussion Fluoride
2021-05-28

Materials and methods Results Discussion Fluoride has multiple effects that contribute to caries prevention. S. mutans is 20- to 40-fold more sensitive to NaF than Lactobacillus casei and seven-fold more sensitive than some species of Actinomyces[10]. There are some specific metabolic effec
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The hepatic cytochrome P CYP superfamily is essential for me
2021-05-28

The hepatic cytochrome P450 (CYP450) superfamily is essential for metabolising foreign chemicals, fatty acids, vitamins, hormones and other compounds and consists of 18 subfamilies (Uno et al., 2012). The number of discovered CYP genes is increasing as a result of intense work with CYP450 gene struc
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First KEGG and GO annotation were conducted for these
2021-05-28

First, KEGG and GO annotation were conducted for these 1251 genes. Then, combining the pathway enrichment analysis, we explored how these genes could function in breast cancer. We discovered that neuroactive ligand-receptor interaction was the most enriched pathway, which comprised numerous G protei
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Introduction Trichloroacetic acid TCA might enhance the acti
2021-05-28

Introduction Trichloroacetic Cyprodime hydrochloride (TCA) might enhance the activation of T cells and disrupt various activities of peripheral T cells [1], which is related to human immune function and reduces human immunity. Trichloroethylene (TCE) has been widely used in many manufacturing indu
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CYP A is involved in the enzymatic
2021-05-28

CYP3A is involved in the enzymatic SBI-0206965 of many therapeutic agents [29]. The activity of BFCOD has been used to monitor CYP3A activity in humans [30], pigs [31], mice [32], and fish [23]. The predominant isoform expressed in the human liver is CYP3A4 [33]. In pigs, CYP3A29 contributes most t
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